| Kyle MacLachlan | Dale Cooper |
| Jay Aaseng | Drunk |
| Dana Ashbrook | Deputy Bobby Briggs |
| Phoebe Augustine | Ronette Pulaski |
| Chrysta Bell | FBI Agent Tammy Preston |
| Jim Belushi | Bradley Mitchum |
| Richard Beymer | Ben Horne |
| Joan Chen | Josie Packard |
| Giselle DaMier | Sandie |
| Eric DaRe | Leo Johnson |
| Don S. Davis | Major Garland Briggs |
| Laura Dern | Diane Evans |
| Jay R. Ferguson | Special Agent Randall Headley |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Robert Forster | Sheriff Frank Truman |
| Nathan Frizzell | Phillip Jeffries |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Robert Knepper | Rodney Mitchum |
| Piper Laurie | Catherine Martell |
| Andrea Watrouse | Mandie |
| Sheryl Lee | Laura Palmer |
| David Lynch | FBI Deputy Director Gordon Cole |
| James Marshall | James Hurley |
| Don Murray | Bushnell Mullins |
| Jack Nance | Pete Martell |
| Walter Olkewicz | Jacques Renault |
| John Pirruccello | Deputy Chad Broxford |
| Kimmy Robertson | Lucy Brennan |
| Carlton Lee Russell | Jumping Man |
| Amy Shiels | Candie |
| Frank Silva | Bob |
| Al Strobel | Phillip Gerard |
| Carel Struycken | The Fireman |
| Jake Wardle | Freddie Sykes |
| Ray Wise | Leland Palmer |
| Nae | Naido |
| Grace Zabriskie | Sarah Palmer |
| Julee Cruise | Julee Cruise |
| Christian Calloway | Black Lodge Woodsman |
| Stewart Strauss | Black Lodge Woodsman |