| Kyle MacLachlan | Dale Cooper |
| Chrysta Bell | FBI Agent Tammy Preston |
| Jim Belushi | Bradley Mitchum |
| Richard Bucher | Man Hit with Bottle |
| Scott Cameron | Throwing Man |
| Jonny Coyne | Polish Accountant |
| Giselle DaMier | Sandie |
| Owain Rhys Davies | Agent Wilson |
| Laura Dern | Diane Evans |
| Josh Fadem | Phil Bisby
(voice) |
| Eamon Farren | Richard Horne |
| Sherilyn Fenn | Audrey Horne |
| Jay R. Ferguson | Special Agent Randall Headley |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Pierce Gagnon | Sonny Jim Jones |
| David Patrick Kelly | Jerry Horne |
| Robert Knepper | Rodney Mitchum |
| Andrea Watrouse | Mandie
(as Andrea Leal) |
| Sheryl Lee | Laura Palmer
(credit only) |
| Jennifer Jason Leigh | Chantal Hutchens |
| Bellina Logan | Female Doctor |
| David Lynch | FBI Deputy Director Gordon Cole |
| Clark Middleton | Charlie |
| Don Murray | Bushnell Mullins |
| Tim Roth | Gary 'Hutch' Hutchens |
| Amy Shiels | Candie |
| J.R. Starr | MC
(as JR Starr) |
| Al Strobel | Phillip Gerard |
| Naomi Watts | Janey-E Jones |
| Eddie Vedder | Edward Louis Severson III |
| Lindsay Lane | Bang Bang Waitress
(uncredited) |
| Alex Reyme | Bang Bang Bar Patron
(uncredited) |