| Kyle MacLachlan | Dale Cooper |
| Jay Aaseng | Drunk |
| Eric Ray Anderson | Bartender |
| Dana Ashbrook | Deputy Bobby Briggs |
| Chrysta Bell | FBI Agent Tammy Preston |
| Monica Bellucci | Monica Bellucci |
| David Bowie | Phillip Jeffries
(archive footage) |
| Robert Broski | Woodsman
(archive footage) |
| Owain Rhys Davies | Agent Wilson |
| Laura Dern | Diane Evans |
| Erica Eynon | Experiment
(archive footage) |
| Jay R. Ferguson | Special Agent Randall Headley |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Robert Forster | Sheriff Frank Truman |
| Nathan Frizzell | Phillip Jeffries
(voice) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Sheryl Lee | Laura Palmer
(archive footage) |
| David Lynch | FBI Deputy Director Gordon Cole |
| Shane Lynch | Megan |
| James Marshall | James Hurley |
| John Paulsen | Trucker |
| John Pirruccello | Deputy Chad Broxford |
| Kimmy Robertson | Lucy Brennan |
| Frank Silva | Bob
(archive footage) |
| J.R. Starr | MC
(as JR Starr) |
| Emily Stofle | Sophie |
| Carel Struycken | The Fireman |
| Jake Wardle | Freddie Sykes |
| Nae | Naido
(as Nae Yuuki) |
| Grace Zabriskie | Sarah Palmer |
| Lissie | Lissie |
| Eric Sullivan | Lissie Band Member |
| Lewis Keller | Lissie Band Member |
| Jesse Siebenberg | Lissie Band Member |
| Anthony Marcacci | |
| Jay Wesley Cochran | Bar Patron
(uncredited) |
| Mélita Toscan du Plantier | Monica Bellucci's Friend
(uncredited) |