| Kyle MacLachlan | Dale Cooper |
| Mädchen Amick | Shelly Briggs |
| Dana Ashbrook | Deputy Bobby Briggs |
| Chrysta Bell | FBI Agent Tammy Preston |
| Jim Belushi | Bradley Mitchum |
| Brent Briscoe | Detective Dave Macklay |
| Catherine E. Coulson | Margaret Lanterman (The Log Lady)
(as Catherine Coulson) |
| Giselle DaMier | Sandie |
| Laura Dern | Diane Evans |
| Josh Fadem | Phil Bisby |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Robert Forster | Sheriff Frank Truman |
| Travis Frost | Boy Playing Catch |
| Balthazar Getty | Red |
| James Grixoni | Deputy Jesse Holcomb |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Caleb Landry Jones | Steven Burnett |
| Laura Kenny | Woman in Car |
| Robert Knepper | Rodney Mitchum |
| Jay Larson | Limo Driver |
| Andrea Watrouse | Mandie
(as Andrea Leal) |
| Sheryl Lee | Laura Palmer
(credit only) |
| Matthew Lillard | William Hastings |
| Peggy Lipton | Norma Jennings |
| Sarah Jean Long | Miriam Sullivan |
| David Lynch | FBI Deputy Director Gordon Cole |
| Don Murray | Bushnell Mullins |
| Priya Niehaus | Sick Girl
(as Priya Diane Niehaus) |
| Cherity Parenzin | Carrie
(as Charity Parenzini) |
| Elias Parenzini | Ralph |
| Linas Phillips | Russ |
| Linda Porter | Lady Slot-Addict |
| Kimmy Robertson | Lucy Brennan
(voice) |
| Marvin Rosand | Toad
(as Marv Rosand) |
| Amanda Seyfried | Rebecca 'Becky' Burnett |
| Amy Shiels | Candie |
| Tom Sizemore | Anthony Sinclair |
| Harry Dean Stanton | Carl Rodd |
| Mary Stofle | Ruth Davenport |
| Al Strobel | Phillip Gerard |
| Lauren Tewes | Neighbor
(as Cynthia Lauren Tewes) |
| Jodi Thelen | Maggie
(as Jodee Thelen) |
| Alicia Witt | Gersten Hayward |
| Christian Calloway | Black Lodge Woodsman
(uncredited) |
| Richard Garcia | Town Person
(uncredited) |
| Smokey Miles | Pianist
(uncredited) |
| David Nieker | Black Lodge Woodsman
(uncredited) |
| Stewart Strauss | Black Lodge Woodsman
(uncredited) |