| Kyle MacLachlan | Dale Cooper |
| Jane Adams | Constance Talbot |
| Stephanie Allynne | Soccer Mom |
| Mädchen Amick | Shelly Briggs |
| Chrysta Bell | FBI Agent Tammy Preston |
| Richard Beymer | Benjamin Horne |
| Brent Briscoe | Detective Dave Macklay |
| Larry Clarke | Detective T. Fusco |
| Laura Dern | Diane Evans |
| Hugh Dillon | Tom Paige |
| Ted Dowling | Farmer |
| Judith Drake | Nurse |
| Eric Edelstein | Detective 'Smiley' Fusco |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Rebecca Field | Another Mom |
| Robert Forster | Sheriff Frank Truman |
| Warren Frost | Doc Will Hayward |
| Ivy George | 5-Year-Old Girl |
| Harry Goaz | Deputy Andy Brennan |
| George Griffith | Ray Monroe |
| Andrea Hays | Heidi |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Ernie Hudson | Colonel Davis |
| Jesse Johnson | Younger Man |
| Ashley Judd | Beverly Paige |
| David Patrick Kelly | Jerry Horne |
| David Koechner | Detective D. Fusco |
| Sheryl Lee | Laura Palmer |
| Peggy Lipton | Norma Jennings |
| David Lynch | FBI Deputy Director Gordon Cole |
| Riley Lynch | Bing |
| Mark Mahoney | Prison Guard |
| Karl Makinen | Inspector Randy Hollister |
| James Morrison | Warden Dwight Murphy |
| Don Murray | Bushnell Mullins |
| Walter Olkewicz | Jean-Michel Renault |
| Adele René | Lieutenant Cynthia Knox |
| Elena Satine | Rhonda |
| Tom Sizemore | Anthony Sinclair |
| Naomi Watts | Janey-E Jones |
| Christophe Zajac-Denek | Ike 'The Spike' Stadtler |
| William Clouter | Sweeping Man |
| Damian Lund | RR Diner Restaurant patron |
| Daniel Nickolaychuk | Lonely Guy at RR Diner |