| Jared Padalecki | Sam Winchester |
| Jensen Ackles | Dean Winchester |
| Jim Beaver | Bobby Singer |
| Alona Tal | Jo Harvelle |
| Faran Tahir | Osiris |
| Julian Christopher | Warren
(as Julian D. Christopher) |
| Emilie Ullerup | Mia |
| Crystal Balint | Jane |
| Stephen Powell | Matthew Hammond |
| Jenn Griffin | AA Sponsor |
| Teach Grant | Christopher Fisher |
| Angelo Renai | Shop Owner |
| Melita Fawcett | Shop Owner's Wife |
| Gerry Waldman | Rabbi |
| Leanne Merrett | Guilty Woman at Bar |
| David Allan Pearson | Detective |
| Thunderbird Dinwiddie | Pamela Barnes
(archive footage) (uncredited) |
| Nicholas Elia | Ben Braeden
(archive footage) (uncredited) |
| Samantha Ferris | Ellen Harvelle
(archive footage) (uncredited) |
| Chris Gauthier | Ronald Reznick
(archive footage) (uncredited) |
| Adrianne Palicki | Jessica Moore
(archive footage) (uncredited) |
| Mark Pellegrino | Lucifer
(voice) (uncredited) |
| Cindy Sampson | Lisa Braeden
(archive footage) (uncredited) |
| Jewel Staite | Amy Pond
(archive footage) (uncredited) |