| Jared Padalecki | Sam Winchester |
| Jensen Ackles | Dean Winchester |
| Alexander Calvert | Jack |
| Rob Benedict | Chuck Shurley /
God |
| Jake Abel | Michael |
| Kimberley Sustad | Betty |
| Mark Pellegrino | Lucifer |
| Kim Rhodes | Sheriff Jody Mills
(voice) |
| Misha Collins | Castiel
(voice) |
| Meena Mann | Casey Ahuja |
| Danneel Ackles | Sister Jo
(archive footage) (uncredited) |
| Curtis Armstrong | Metatron
(archive footage) (uncredited) |
| Jim Beaver | Bobby Singer
(archive footage) (uncredited) |
| Briana Buckmaster | Donna Hanscum
(archive footage) (uncredited) |
| Osric Chau | Kevin Tran
(archive footage) (uncredited) |
| Ruth Connell | Rowena MacLeod
(archive footage) (uncredited) |
| Felicia Day | Charlie Bradbury
(archive footage) (uncredited) |
| Kurt Fuller | Zachariah
(archive footage) (uncredited) |
| Rachel Miner | The Empty /
Meg
(archive footage) (uncredited) |
| Jeffrey Dean Morgan | John Winchester
(archive footage) (uncredited) |
| Emily Perkins | Becky Rosen
(archive footage) (uncredited) |
| DJ Qualls | Garth Fitzgerald IV
(archive footage) (uncredited) |
| Julian Richings | Death
(archive footage) (uncredited) |
| Mark Sheppard | Crowley
(archive footage) (uncredited) |
| Samantha Smith | Mary Winchester
(archive footage) (uncredited) |
| Emily Swallow | Amara
(archive footage) (uncredited) |
| Steven Williams | Rufus Turner
(archive footage) (uncredited) |