| Dominic Purcell | Lincoln 'Linc' Burrows |
| Wentworth Miller | Michael Scofield |
| Amaury Nolasco | Fernando Sucre |
| Marshall Allman | LJ Burrows |
| Paul Adelstein | Paul Kellerman |
| Robert Knepper | Theodore 'T-Bag' Bagwell |
| Rockmond Dunbar | Benjamin Miles 'C-Note' Franklin |
| Sarah Wayne Callies | Sara Tancredi |
| William Fichtner | Alexander 'Alex' Mahone |
| Lane Garrison | David 'Tweener' Apolskis |
| Silas Weir Mitchell | Charles 'Haywire' Patoshik |
| John Heard | Governor Frank Tancredi |
| Reggie Lee | Bill Kim |
| Diana Scarwid | Jeanette Owens |
| Alexandra Lydon | Ann Owens |
| Jason Davis | FBI Agent Wheeler |
| Kristin Malko | Debra Jean Belle |
| Joe Inscoe | Lyle Sands |
| Wilbur Fitzgerald | Bruce Bennett |
| Steven Chester Prince | Agent Blondie |
| Steve Coulter | Detective |
| Tom Clark | Keith |
| Ryan Rutledge | Krance |
| Jodie Moore | Proprietor |
| Akai Draco | C.O. |
| Aleisha Force | Governor's Assistant |
| Clarice Tinsley | News Host |
| Damon Carney | Mysterious Man |
| Robert F. Cawley | Hospital Patient |
| Riley Chambers | Arizona Department of Corrections Officer |
| Steve M. Clark | Illinois State Police |
| Craig Cole | FBI Agent Eric Houle |
| Jimmy Daniels | Oscar Shales |
| Jamie Dugger | Woman |
| Starla Osburn | Pedestrian |
| Jeffrey S. Parkerson | Federal Agent |