| Dominic Purcell | Lincoln 'Linc' Burrows |
| Wentworth Miller | Michael Scofield |
| Robin Tunney | Veronica Donovan |
| Amaury Nolasco | Fernando Sucre |
| Marshall Allman | LJ Burrows |
| Wade Williams | Brad Bellick |
| Paul Adelstein | Paul Kellerman |
| Robert Knepper | Theodore 'T-Bag' Bagwell |
| Rockmond Dunbar | Benjamin Miles 'C-Note' Franklin |
| Sarah Wayne Callies | Sara Tancredi |
| Muse Watson | Charles Westmoreland |
| Michelle Forbes | Samantha Brinker |
| Frank Grillo | Nick Savrinn |
| Lane Garrison | David 'Tweener' Apolskis |
| Silas Weir Mitchell | Charles 'Haywire' Patoshik |
| Matt DeCaro | Corrections Officer Roy Geary |
| Patricia Wettig | Vice President Caroline Reynolds |
| Stacy Keach | Warden Henry Pope |
| DuShon Monique Brown | Nurse Katie Welch |
| Daniel Allar | Avocado |
| Cynthia Kaye McWilliams | Kacee Franklin |
| Kirk Anderson | Annie the Tranny |
| Phillip Edward Van Lear | Louis Patterson |
| Christian Stolte | Corrections Officer Keith Stolte |
| Jennifer Joan Taylor | Becky |
| Ray Austin | Sally Port C.O. |
| Javon Johnson | Ad Seg C.O. |
| Christine Devine | News Anchor |
| Rolando Boyce | Cop #1 |
| Brian King | Orderly |
| Zachary Laoutides | Prisoner |
| David Dino Wells Jr. | Trumpets Right-hand Man |
| James Zahn | Psych Ward Inmate |