| Dominic Purcell | Lincoln 'Linc' Burrows |
| Wentworth Miller | Michael Scofield |
| Robin Tunney | Veronica Donovan |
| Peter Stormare | John Abruzzi |
| Amaury Nolasco | Fernando Sucre |
| Wade Williams | Brad Bellick |
| Paul Adelstein | Paul Kellerman |
| Robert Knepper | Theodore 'T-Bag' Bagwell |
| Sarah Wayne Callies | Sara Tancredi |
| Muse Watson | Charles Westmoreland |
| Michelle Forbes | Samantha Brinker |
| Rockmond Dunbar | Benjamin Miles 'C-Note' Franklin |
| Danny McCarthy | Daniel Hale |
| Frank Grillo | Nick Savrinn |
| Mike Jones | Darius |
| Matt DeCaro | Corrections Officer Roy Geary |
| Patricia Wettig | Vice President Caroline Reynolds |
| Stacy Keach | Warden Henry Pope |
| Phillip Edward Van Lear | Louis Patterson |
| Tom McElroy | Reverend Mailor |
| Jenny Kern | Allison Hale |
| DuShon Monique Brown | Nurse Katie Welch |
| Karin Anglin | Angela West |
| Christian Stolte | Corrections Officer Keith Stolte |
| Javon Johnson | C.O. #1 |
| Lea Coco | C.O. #2 |
| Eric Lane | Spider |
| Helena Klevorn | Dede Franklin |
| Cynthia Kaye McWilliams | Kacee Franklin |
| Jacqueline Williams | Special Agent Tangrin |
| David Gee | Producer |
| Joey Honsa | Holly - Reporter |
| Kurt Naebig | C.O. Kurt |
| Tory O. Davis | Orderly |
| Manny Sosa | Janitor |
| Chuck Stubbings | EMT #1 |
| Terry Berner | Electrician |
| Nick Doetsch | Licoln's double |
| Dennis Janiske | Inmate |
| Tim Krueger | Prosecutor |
| Zachary Laoutides | Prisoner |
| Joseph A Olivera | Corrections Officer |
| Angelina Riposta | Pedestrian |
| Ryan Salzwedel | EMT #2 |
| John Stacho | Protestor Fox River Penitentiary |
| Bruce A. Young | C.O. #2 in clinic |