| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Cote de Pablo | Ziva David |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Brian Dietzen | Jimmy Palmer |
| Sarah Jane Morris | NCIS Special Agent Erica Jane 'EJ' Barrett |
| Jeffrey Johnson | Commander Nelson Tunney |
| Cara Pifko | Kelsey Donner |
| John Ales | Miles Hogan |
| Terrell Tilford | Navy Captain Jack Painter |
| Alicia Ziegler | Marine Corporal Jameson |
| Terry Woodberry | Bates |
| Jason Sarcinelli | Navy Petty Officer Eric Donner |
| Kimberly Alexander | Navy Lieutenant Junior Grade Lauren Ross |
| Alimi Ballard | NCIS Special Agent Gayne Levin |
| Jerald Garner | SECNAV Davenport PSA |
| Milauna Jackson | Emma Park |
| Matthew Willig | NCIS Special Agent Simon Cade |