| Mark Harmon | Leroy Jethro Gibbs |
| Sean Murray | Timothy McGee |
| Wilmer Valderrama | Nick Torres |
| Emily Wickersham | Ellie Bishop |
| Maria Bello | Jack Sloane |
| Brian Dietzen | Jimmy Palmer |
| Diona Reasonover | Kasie Hines |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Don Lake | Navy Captain Phillip Brooks |
| Kate Orsini | FBI Special Agent Isabel Monet |
| Emily Rose | FBI Special Agent Anna Jenlowe |
| Amielynn Abellera | Navy Lieutenant Kaitlyn Holcomb |
| Daryl C. Brown | Navy Commander Lovinder Prasad |
| Ian Gregory | FAA Security Specialist Horace Barnes |
| Rich Ceraulo Ko | North Carolina State Trooper Muncie Tyson |
| Bo Kane | North Carolina State Trooper William Forman |
| Joe Spano | T.C. Fornell |
| Jill Czarnowski | NCIS Tech Susan Ottsley |