| Mark Harmon | Leroy Jethro Gibbs |
| Michael Weatherly | Anthony DiNozzo |
| Pauley Perrette | Abby Sciuto |
| Sean Murray | Timothy McGee |
| Brian Dietzen | Jimmy Palmer |
| Emily Wickersham | Ellie Bishop |
| Rocky Carroll | Leon Vance |
| David McCallum | Donald Mallard |
| Eric Matheny | Former NCIS Agent Kip Klugman |
| Gregory Scott Cummins | Kobe 'Beef' Carver |
| Heather Mazur | Laura Strike-DePalma |
| Edward Fordham Jr. | Burly Man a.k.a. 'Lump' |
| Brett DelBuono | Navy Seaman Apprentice Brian Dokes |
| Diandra Lyle | Alexandria P.D. Karen Stradivarius |
| Leslie Hope | Secretary of the Navy Sarah Porter |