| Clark Gregg | Phil Coulson |
| Ming-Na Wen | Melinda May |
| Brett Dalton | Grant Ward |
| Chloe Bennet | Daisy 'Skye' Johnson |
| Iain De Caestecker | Leo Fitz |
| Elizabeth Henstridge | Jemma Simmons |
| Nick Blood | Lance Hunter |
| Adrianne Palicki | Bobbi Morse |
| Henry Simmons | Alphonso 'Mack' Mackenzie |
| Luke Mitchell | Lincoln Campbell
(credit only) |
| Peter MacNicol | Professor Elliot Randolph |
| James Hong | William May |
| Alex Hyde-White | Lord Thornally |
| Spencer Treat Clark | Werner von Strucker |
| Blair Underwood | Andrew Garner |
| Daz Crawford | Kebo |
| Daniel Wolfe | Lord Manzini |
| Piers Stubbs | Younger Lord |
| Ashley Caple | Alexander's Friend on Yacht
(uncredited) |
| Mark Casimir Dyniewicz Jr. | 1800's Nobleman
(uncredited) |
| Riley Go | Young Melinda May
(uncredited) |
| Trevor Snarr | Norway Prison Guard Huntington
(uncredited) |