| Josh Radnor | Ted Mosby |
| Jason Segel | Marshall Eriksen |
| Cobie Smulders | Robin Scherbatsky |
| Neil Patrick Harris | Barney Stinson |
| Alyson Hannigan | Lily Aldrin |
| Paul Shaffer | Self |
| Alex Trebek | Self |
| Abby Elliott | Jeanette |
| James Van Der Beek | Simon |
| Alan Thicke | Alan Thicke |
| Dave Thomas | Chuck Gerussi |
| Jason Priestley | Self |
| Geddy Lee | Self |
| Steven Page | Self |
| Dave Coulier | Self |
| Luc Robitaille | Self |
| k.d. lang | k.d. lang |
| Craig Lee Thomas | Turk |
| Corey Krueger | Gordie |
| Jim Forbes | U.T.T. Narrator
(voice) (as James Jude Forbes) |
| Chuck Lacey | Old Man |
| Jeff T. Buco | Canadian Mountie
(uncredited) |
| Richard Lawrence | Royal Canadian Mounted Police
(uncredited) |
| Dana Lee | Grungy Slacker
(uncredited) |
| Rebecca Newton | Crew Cut Girl
(uncredited) |
| Bob Saget | Narrator
(voice) (uncredited) |