| Ben McKenzie | James Gordon |
| Donal Logue | Harvey Bullock |
| David Mazouz | Bruce Wayne |
| Morena Baccarin | Leslie Thompkins |
| Sean Pertwee | Alfred Pennyworth |
| Robin Lord Taylor | Oswald Cobblepot /
Penguin |
| Erin Richards | Barbara Kean
(credit only) |
| Camren Bicondova | Selina Kyle
(credit only) |
| Cory Michael Smith | Edward Nygma /
The Riddler |
| Jessica Lucas | Tabitha Galavan |
| Chris Chalk | Lucius Fox
(credit only) |
| Drew Powell | Solomon Grundy |
| Crystal Reed | Sofia Falcone
(credit only) |
| Alexander Siddig | Ra's al Ghul
(credit only) |
| Cameron Monaghan | Jerome Valeska /
Jeremiah Valeska |
| Nathan Darrow | Victor Fries /
Mr. Freeze |
| Benedict Samuel | Jervis Tetch |
| Michelle Veintimilla | Bridgit Pike /
Firefly |
| David W. Thompson | Jonathan Crane /
Scarecrow |
| Steven Hauck | Headmaster |
| Kelcy Griffin | Harper |
| Francesca Root-Dodson | Ecco |
| James Lurie | Allan Hayes |
| Jonathan Rentler | Carl |
| Jason Yudoff | Lars |
| John Garrett Greer | Businessman |
| Lina Lee | Receptionist |
| Claudia Robinson | Lady |
| Arami Kramer | Lila |
| Jack Hammel | Dr. Thompkins' Goon |
| Dante Jayce | The Lowlife Biker
(uncredited) |
| Allison Lahikainen | Narrows Punk
(uncredited) |
| Kevin D. McGee | GCPD Officer
(uncredited) |
| Matt Murray | Punk on catwalk
(uncredited) |
| Stuart Schnitzer | Core Desk Sergeant
(uncredited) |
| Justin Whang | Member of Joker's Army
(uncredited) |
| Kelly P. Williams | Gotham City Police
(uncredited) |