| Anna Torv | Olivia Dunham /
Alternate Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles /
Alternate Phillip Broyles |
| Blair Brown | Alternate Nina Sharp |
| Jasika Nicole | Astrid Farnsworth /
Alternate Astrid Farnsworth |
| Seth Gabel | Lincoln Lee /
Alternate Lincoln Lee |
| John Noble | Dr. Walter Bishop |
| Jared Harris | David Robert Jones |
| Curtis Harris | Christopher Broyles |
| Carolyn Anderson | Red-Headed Woman |
| Josh Blacker | MP |
| Chilton Crane | Alt Lincoln's Mother |
| Karen Holness | Alternate Diane Broyles |
| Stephen Huszar | Sgt. Wheeler |
| David Lewis | Brian Bauer |
| Broadus Mattison | Guard at Bridge |
| Adrian Neblett | Cab Driver |
| Wesley Salter | Man in Suit |
| Daryl Shuttleworth | Alt Lincoln's Father |
| Ryan Swanson | Clerk |
| Scott Vickaryous | Jeremy |
| Jocelyn Panton | Anne
(uncredited) |