| Anna Torv | Olivia Dunham /
Alternate Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles /
Alternate Phillip Broyles |
| Blair Brown | Alternate Nina Sharp |
| Jasika Nicole | Astrid Farnsworth /
Alternate Astrid Farnsworth |
| Seth Gabel | Lincoln Lee /
Alternate Lincoln Lee |
| John Noble | Dr. Walter Bishop /
Walternate |
| Orla Brady | Alternate Elizabeth Bishop |
| Jared Harris | David Robert Jones |
| Michelle Krusiec | Nadine Park |
| Chaz Chamberlain | ND Agent #1 |
| Jarod Joseph | FBI Agent Tim |
| Achara Kirk | ND Agent #2 |
| Harrison MacDonald | Teenage Boy |
| Henry Mah | Nurse
(as Henry J. Mah) |
| Edwin Perez | Techie |
| Maja Aro | Shapeshifter in Tank
(as Maja Stace-Smith) |
| Desiree Zurowski | Doctor |