| Anna Torv | Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles |
| Blair Brown | Nina Sharp |
| Jasika Nicole | Alternate Astrid Farnsworth |
| John Noble | Dr. Walter Bishop |
| Seth Gabel | Alternate Lincoln Lee |
| Amy Madigan | Marilyn Dunham |
| Andre Royo | Henry Arliss Higgins |
| Philip Winchester | Frank Stanton |
| Ryan McDonald | Alternate Brandon Fayette |
| Gerard Plunkett | Senator James Van Horn |
| Gabrielle Rose | Dr. Anderson |
| Kirk Acevedo | Alternate Charlie Francis |
| Juliana Wimbles | Nurse Melissa |
| Lucas Wolf | Male Nurse |
| Darryl Scheelar | MP |
| Fraser Corbett | Second Man |
| Viv Leacock | Cop |
| Crystal Balint | ND Agent |
| Gino Cocomello | Gas Station Attendant |