| Anna Torv | Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Lance Reddick | Phillip Broyles |
| Blair Brown | Nina Sharp
(credit only) |
| Jasika Nicole | Astrid Farnsworth |
| John Noble | Dr. Walter Bishop |
| Ravil Isyanov | Tomas |
| JR Bourne | Mystery Man |
| Gerard Plunkett | Senator James Van Horn |
| Blaine Anderson | CSI Officer |
| Jeanettea Antonio | Hospital Security
(as Jeanetta Antonio) |
| Charisse Baker | Girl's Mother |
| Megan Charpentier | Little Girl |
| Chris Eastman | CSI Investigator |
| Michelle Harrison | Natalie Dancik |
| Karen Holness | Diane Broyles |
| Linnea Johnson | Nurse #1 |
| Linda Ko | Nurse Darla |
| D. Neil Mark | ND Agent #2 |
| Johanna Marlowe | Nurse Maxine |
| Matteo Mazziotti | Boy in Restaurant |
| Dean McKenzie | Wife's Boyfriend
(as Dean Monroe McKenzie) |
| Angela Moore | Another Nurse |
| Yaroslav Poverlo | Alex Koslov |
| Chris Shields | ND Agent |
| Veena Sood | HR Head |
| Joe Towne | Randy Dancik |
| Jeny Cassady | Dying Patient
(uncredited) |
| Daryl Schwartzberg | Flight Attendant
(uncredited) |