| Anna Torv | Olivia Dunham |
| Joshua Jackson | Peter Bishop |
| Kirk Acevedo | Charlie Francis |
| Lance Reddick | Phillip Broyles |
| Blair Brown | Nina Sharp |
| Jasika Nicole | Astrid Farnsworth |
| Mark Valley | John Scott |
| John Noble | Dr. Walter Bishop |
| Jared Harris | David Robert Jones |
| Paul Fitzgerald | Ryan Eastwick |
| Chance Kelly | Mitchell Loeb |
| James Frain | Mr. Kohl |
| Angel David | Raul Lugo |
| Vince Cupone | Evan McNeil
(as Vince Capone) |
| Brian Cromwell | Robert Norton |
| Brian Slaten | Man #1 |
| Sean Mahon | Bartender
(as Sean F. Mahon) |
| Belinda Sinclair | Bar Patron |
| Jennifer Van Dyck | Chief Technician |
| Rosa Arredondo | Susan Lugo |
| Amir Arison | Dr. Bruce Miller |
| Andre B. Blake | Ed 'Smitty' Smith
(as Andre Blake) |
| Karl Kenzler | Special Agent Martin |
| Village | Bradley
(as Village Dumetz) |
| Frank Deal | Bank Manager Grimes |
| Emily Brownell | H.I. Worker |
| R.J. Foster | Surveyor #1
(as RJ Foster) |
| Greg Schmalbach | Surveyor #2 |
| Eric Chan | Surveyor #4 |
| Matthew Quinn Martin | ND Agent
(as Matthew Martin) |
| Darby Lynn Totten | ND Agent #2
(as Darby Totten) |
| James Hook | ND Agent #3 |
| Michael Cerveris | The Observer
(uncredited) |
| David Shumbris | Man #2
(uncredited) |