| Neil Patrick Harris | Count Olaf |
| Patrick Warburton | Lemony Snicket |
| Malina Weissman | Violet Baudelaire |
| Louis Hynes | Klaus Baudelaire |
| K. Todd Freeman | Arthur Poe |
| Presley Smith | Sunny Baudelaire |
| Lucy Punch | Esmé Squalor |
| Dylan Kingwell | Quigley Quagmire |
| Allison Williams | Kit Snicket |
| Roger Bart | Vice Principal Nero |
| Tony Hale | Jerome Squalor |
| Max Greenfield | Denouement Brothers |
| Richard E. Grant | Man with Beard But No Hair |
| Beth Grant | Woman with Hair But No Beard |
| Morena Baccarin | Beatrice |
| Kitana Turnbull | Carmelita Spats |
| Kerri Kenney | Babs |
| Carol Mansell | Elder Jemma |
| Joan Cusack | Justice Strauss |
| Eric Keenleyside | Fire Chief |
| Lauren McGibbon | Perky Volunteer |
| Darcey Johnson | Trolleyman |
| Rob LaBelle | Taxi Driver |
| Timothy Webber | Jimmy |
| Matty Cardarople | Henchperson of Indeterminate Gender |
| Peter Brown | Hotel Guest |
| Cynthia Mendez | Volunteer Singer #4 |
| Arielle Tuliao | Volunteer Singer #1 |