| Michael C. Hall | Dexter Morgan |
| Jennifer Carpenter | Debra Morgan |
| Desmond Harrington | Det. Joseph 'Joey' Quinn |
| C.S. Lee | Vince Masuka |
| Luna Lauren Velez | Capt. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Sgt. Angel Batista |
| James Remar | Harry Morgan |
| Colin Hanks | Travis Marshall |
| Edward James Olmos | Prof. James Gellar |
| Geoff Pierson | Deputy Chief Tom Matthews |
| Aimee Garcia | Jamie Batista |
| Billy Brown | Det. Mike Anderson |
| Josh Cooke | Louis Greene |
| Kyle Davis | Steve Dorsey |
| Rya Kihlstedt | Dr. Michelle Ross |
| Alex Hyde-White | Dr. Trent Casey |
| Evan George Kruntchev | Harrison Morgan |
| Luke Andrew Kruntchev | Harrison Morgan |
| Michael Patrick McGill | Troy |
| J. Downing | Mr. Morris |
| Judson Mills | Floyd |
| Elizabeth Tobias | Sister Daniels |
| Sheila Shaw | Connie |
| Niko Posey | Concerned Citizen |
| Whitney Anderson | Young Woman |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |
| Kimberly Langley | Travel Agent
(uncredited) |