| Michael C. Hall | Dexter Morgan |
| Jennifer Carpenter | Debra Morgan |
| Desmond Harrington | Det. Joseph 'Joey' Quinn |
| C.S. Lee | Vince Masuka |
| Luna Lauren Velez | Capt. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Sgt. Angel Batista |
| James Remar | Harry Morgan |
| Colin Hanks | Travis Marshall |
| Edward James Olmos | Prof. James Gellar |
| Christian Camargo | Brian Moser |
| Aimee Garcia | Jamie Batista |
| Billy Brown | Det. Mike Anderson |
| Molly Parker | Lisa Marshall |
| Josh Cooke | Louis Greene |
| Brando Eaton | Jonah Mitchell |
| Scott Michael Campbell | Norm |
| Cherilyn Wilson | Laci |
| Evan George Kruntchev | Harrison Morgan |
| Luke Andrew Kruntchev | Harrison Morgan |
| Lacey Beeman | Holly Benson |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |
| Vanessa Marano | Rebecca Mitchell
(uncredited) |