| Michael C. Hall | Dexter Morgan |
| Jennifer Carpenter | Debra Morgan |
| Desmond Harrington | Det. Joseph 'Joey' Quinn |
| C.S. Lee | Vince Masuka |
| Luna Lauren Velez | Capt. Maria LaGuerta
(as Lauren Velez) |
| David Zayas | Sgt. Angel Batista |
| James Remar | Harry Morgan |
| Colin Hanks | Travis Marshall |
| Yasiin Bey | Brother Sam |
| Edward James Olmos | Prof. James Gellar |
| Christian Camargo | Brian Moser |
| Aimee Garcia | Jamie Batista |
| Billy Brown | Det. Mike Anderson |
| Molly Parker | Lisa Marshall |
| Mariana Klaveno | Carissa Porter |
| Josh Cooke | Louis Greene |
| Rya Kihlstedt | Dr. Michelle Ross |
| Luke Andrew Kruntchev | Harrison Morgan |
| Germaine De Leon | Nick |
| Justin Huen | Diego |
| Lacey Beeman | Holly Benson |
| Nick McCallum | Junkie |
| Lauren Rose Lewis | Cindy |
| Caitlin Carmichael | Ella |
| Gabreon Womack | Liam |
| Neto DePaula Pimenta | Leo Hernandez |
| Tanya R. Barnes | Market Vendor
(uncredited) |
| Bruce Holman | Det. Michael Soderquist
(uncredited) |
| Jet Set Hudson | Extra
(uncredited) |
| Kimberly Langley | Forensic Technician in Leo's House
(uncredited) |