| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne
(credit only) |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings
(credit only) |
| James Marshall | James Hurley
(credit only) |
| Everett McGill | Big Ed Hurley
(credit only) |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran |
| Joan Chen | Jocelyn Packard
(credit only) |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Kenneth Welsh | Windom Earle |
| Billy Zane | John Justice Wheeler |
| Ian Buchanan | Dick Tremayne |
| Heather Graham | Annie Blackburn |
| Robyn Lively | Lana Budding Milford |
| David Lynch | FBI Regional Bureau Chief Gordon Cole |
| Wendy Robie | Nadine Hurley |
| Don S. Davis | Maj. Garland Briggs
(as Don Davis) |
| Gary Hershberger | Mike Nelson |
| Mary Jo Deschanel | Eileen Hayward |
| John Boylan | Mayor Dwayne Milford |
| Ted Raimi | Heavy Metal Youth |
| John Charles Sheeham | Bellman
(as John Charles Sheehan) |