| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward
(credit only) |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran
(voice) |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Kenneth Welsh | Windom Earle |
| Billy Zane | John Justice Wheeler |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| David Patrick Kelly | Jerry Horne |
| Chris Mulkey | Hank Jennings |
| Wendy Robie | Nadine Hurley |
| David Warner | Thomas Eckhardt |
| Dan O'Herlihy | Andrew Packard |
| Michael J. Anderson | Man From Another Place |
| Ron Blair | Randy St. Croix |
| Frank Silva | Bob |