| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Chris Mulkey | Hank Jennings |
| Ian Buchanan | Dick Tremayne |
| Clarence Williams III | FBI Agent Roger Hardy |
| James Booth | Ernie Niles |
| David Duchovny | DEA Agent Dennis |
| Wendy Robie | Nadine Hurley |
| Charlotte Stewart | Betty Briggs |
| Gary Hershberger | Mike Nelson |
| Tony Jay | Dougie Milford |
| Robyn Lively | Lana Budding Milford |
| Annette McCarthy | Evelyn Marsh |
| Royce D. Applegate | Rev. Clarence Brocklehurst |
| Ron Taylor | Coach Wingate |
| Dan O'Herlihy | Andrew Packard |
| John Boylan | Mayor Dwayne Milford |
| Catherine E. Coulson | The Log Lady |
| Jill Engels | Trudy |
| Joshua Harris | Nicky |
| David Lynch | FBI Regional Bureau Chief Gordon Cole |
| Kenneth Welsh | Windom Earle |