| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley
(credit only) |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Grace Zabriskie | Sarah Palmer |
| Chris Mulkey | Hank Jennings |
| Ian Buchanan | Dick Tremayne |
| James Booth | Ernie Niles |
| Jane Greer | Vivian Smythe Niles |
| Clarence Williams III | FBI Agent Roger Hardy |
| Gavan O'Herlihy | RCMP Officer Preston King |
| Wendy Robie | Nadine Hurley |
| Tony Jay | Dougie Milford |
| Don S. Davis | Maj. Garland Briggs
(as Don Davis) |
| Mary Jo Deschanel | Eileen Hayward |
| Don Calfa | Vice Principal Greege |
| Michael Parks | Jean Renault |
| John Boylan | Mayor Dwayne Milford |
| Lisa Cloud | P. E. Teacher |
| Tiffany Muxlow | Cheerleader |
| Susan Sundholm | Samantha |