| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran
(credit only) |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard
(credit only) |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Maddy Ferguson |
| Grace Zabriskie | Sarah Palmer |
| Wendy Robie | Nadine Hurley |
| Al Strobel | Phillip Michael Gerard |
| David Lynch | FBI Regional Bureau Chief Gordon Cole |
| Piper Laurie | Mr. Tojamura
(as Fumio Yamaguchi) |
| Gary Hershberger | Mike Nelson |
| Catherine E. Coulson | The Log Lady |
| Julee Cruise | Singer |
| Carel Struycken | Giant |
| Hank Worden | Waiter |
| Dave Bean | Sheriff's Deputy
(uncredited) |
| Kellie Lumb | USO Girl
(uncredited) |
| Chris Morrissey | Barfly
(uncredited) |
| Frank Silva | Bob
(uncredited) |