| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Kimmy Robertson | Lucy Moran |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Maddy Ferguson |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Ian Buchanan | Dick Tremayne |
| Lenny von Dohlen | Harold Smith |
| Wendy Robie | Nadine Hurley |
| Don Amendolia | Emory Battis |
| Victoria Catlin | Blackie O'Reilly |
| Michael Parks | Jean Renault |
| Galyn Görg | Nancy |
| Al Strobel | Phillip Michael Gerard |
| Phoebe Augustine | Ronette Pulaski |
| Mak Takano | Asian Man |
| Jennifer Aquino | Eolani Jacoby |
| Walter Olkewicz | Jacques Renault |
| Connie Woods | The New Girl at One-Eyed Jacks |