| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Maddy Ferguson |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Chris Mulkey | Hank Jennings |
| David Patrick Kelly | Jerry Horne |
| Don S. Davis | Maj. Garland Briggs |
| Charlotte Stewart | Betty Briggs |
| Don Amendolia | Emory Battis |
| Catherine E. Coulson | The Log Lady |
| Jill Engels | Trudy |
| Brian Straub | Einar Thorson |
| Mary Stavin | Heba |
| Lance Davis | Chet (segment 'Invitation To Love') |
| Rick Giolito | Montana (segment 'Invitation To Love') |
| Peter Michael Goetz | Jared (segment 'Invitation To Love') |
| Lisa Ann Cabasa | Jenny |
| Grace Zabriskie | Sarah Palmer |