| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Maddy Ferguson /
Laura Palmer |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Grace Zabriskie | Sarah Palmer |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Walter Olkewicz | Jacques Renault
(as Walter Oklewicz) |
| Royce D. Applegate | Rev. Clarence Brocklehurst |
| Jed Mills | Wilson Mooney |
| Wendy Robie | Nadine Hurley |
| Kimmy Robertson | Lucy Moran |
| Gary Hershberger | Mike Nelson |
| Don S. Davis | Maj. Garland Briggs
(as Don Davis) |
| Charlotte Stewart | Betty Briggs |
| Robert Bauer | Johnny Horne |
| Catherine E. Coulson | The Log Lady |
| Jill Engels | Trudy
(as Jill Rogosheske) |
| Brett Vadset | Joey Paulson |
| Clay Wilcox | Bernard Renault |
| Erika Anderson | Emerald (segment 'Invitation To Love') /
Jade (segment 'Invitation To Love') |
| Lance Davis | Chet (segment 'Invitation To Love') |
| Peter Michael Goetz | Jared (segment 'Invitation To Love') |
| Jan D'Arcy | Sylvia Horne
(voice) (uncredited) |
| Rick Giolito | Montana - 'Invitation To Love'
(uncredited) |
| Eve Hands | Woman Shopper
(uncredited) |