| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Laura Palmer |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Grace Zabriskie | Sarah Palmer |
| David Patrick Kelly | Jerry Horne |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Victoria Catlin | Blackie O'Reilly |
| Wendy Robie | Nadine Hurley |
| Kimmy Robertson | Lucy Moran |
| Jan D'Arcy | Sylvia Horne |
| Mary Jo Deschanel | Eileen Hayward |
| Gary Hershberger | Mike Nelson |
| Michael J. Anderson | Man From Another Place |
| Robert Bauer | Johnny Horne |
| Kim Lentz | Bartender |
| Frank Silva | Bob |
| Charlie Spradling | Swabbie |
| Al Strobel | One-Armed Man |
| Jill Engels | Trudy Chelgren |
| Clay Wilcox | Bernard Renault |
| Connie Woods | New Girl at One Eyed Jacks |