| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson
(as Madchen Amick) |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Eric DaRe | Leo Johnson
(as Eric Da Re) |
| Harry Goaz | Deputy Andy Brennan |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Sheryl Lee | Laura Palmer |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Grace Zabriskie | Sarah Palmer |
| Don S. Davis | Maj. Garland Briggs
(as Don Davis) |
| Mary Jo Deschanel | Eileen Hayward |
| Charlotte Stewart | Betty Briggs |
| Gary Hershberger | Mike Nelson |
| Wendy Robie | Nadine Hurley |
| Kimmy Robertson | Lucy Moran |
| Catherine E. Coulson | The Log Lady |
| Alan Ogle | Janek Pulaski |
| Michelle Milantoni | Suburbis Pulaski
(as Michele Milantoni) |
| Jill Engels | Trudy
(as Jill Rogosheske) |
| Al Strobel | One-Armed Man |
| Laurel White | Nurse Greta
(as Laurel Anne White) |
| Frank Silva | Bob
(uncredited) |