| Kyle MacLachlan | Special Agent Dale Cooper |
| Michael Ontkean | Sheriff Harry S. Truman |
| Mädchen Amick | Shelly Johnson |
| Dana Ashbrook | Bobby Briggs |
| Richard Beymer | Benjamin Horne |
| Lara Flynn Boyle | Donna Hayward |
| Sherilyn Fenn | Audrey Horne |
| Warren Frost | Dr. Will Hayward |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Jack Nance | Pete Martell |
| Ray Wise | Leland Palmer |
| Joan Chen | Jocelyn Packard |
| Piper Laurie | Catherine Martell |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Eric DaRe | Leo Johnson |
| Mary Jo Deschanel | Eileen Hayward |
| Harry Goaz | Deputy Andy Brennan |
| Gary Hershberger | Mike Nelson |
| Michael Horse | Deputy Tommy 'Hawk' Hill |
| Grace Zabriskie | Sarah Palmer |
| Troy Evans | George Wolchezk |
| John Boylan | Dwayne Milford |
| Rodney Harvey | Biker Scotty |
| Sheryl Lee | Laura Palmer |
| Robert Davenport | Johnny Horne |
| Jan D'Arcy | Sylvia Horne |
| Kimmy Robertson | Lucy Moran |
| Jessica Wallenfels | Harriet Hayward |
| Wendy Robie | Nadine Hurley |
| Don S. Davis | Maj. Garland Briggs |
| Charlotte Stewart | Betty Briggs |
| Roberta Maguire | Maria Pulaski |
| Phoebe Augustine | Ronnette Pulaski |
| Frank Roberts | Father Hutchings |
| Brett Vadset | Joey |
| David Wasman | Gilman White |
| Jane Jones | Margaret Honeycutt |
| Tawnya Pettiford-Wates | Dr. Shelvy |
| Shelley Henning | Alice Brady |
| Dorothy Roberts | Mrs. Jackson |
| Julee Cruise | Girl Singer |
| Arnie Stenseth | Sven Jorgenson |
| Andrea Hays | Heidi |
| Rick Tutor | Janek Pulaski |
| Marjorie Nelson | Janice Hogan |
| Ben DiGregorio | Max Hartman |
| Diane Caldwell | Hotel Concierge Julie Duvic |
| Catherine E. Coulson | The Log Lady |
| Al Strobel | One-Armed Man |
| David Chadwick | Harry Wilson |
| Laurel White | Nurse Greta |
| Joey Altruda | Road House Band Member |
| Michael J. Anderson | Man From Another Place (international version only) |
| Steven Hodges | Road House Band Member |
| Smokey Hormel | Road House Band Member |
| David Lynch | Reflection of the Man in Headphones in Police Station |
| Frank Silva | Bob |
| Joseph 'Simon' Szeibert | Road House Band Member |
| William Ungerman | Road House Band Member |