| Joe Mantegna | David Rossi |
| Shemar Moore | Derek Morgan |
| Matthew Gray Gubler | Dr. Spencer Reid |
| A.J. Cook | Jennifer Jareau |
| Kirsten Vangsness | Penelope Garcia |
| Jeanne Tripplehorn | Alex Blake |
| Thomas Gibson | Aaron Hotchner |
| Brad Dourif | Adam Rain |
| Mark Povinelli | Mr. Conrad |
| Beth Riesgraf | Dr. Maeve Donovan |
| Hanna Hall | Connie Foster |
| Suzanne Krull | Dr. Sarah Glenn |
| Hans Howes | Mr. James |
| Jay Hayden | Bobby Putnam |
| Sean Whalen | Tucker White |
| Gary Carlos Cervantes | Detective Martin
(as Gary Cervantes) |
| Kurtis Mast | Byron Sadler
(as Kurtis James Erwin) |
| Michael Boucher | Evan Davis
(as Michael Charles Boucher) |
| Matt Kirkwood | George Small |
| John Hawkinson | Alex Rain |
| Steve Suh | CSI Tech |
| Maya Stojan | Hostess |
| Jake Brennan | Matt Small |
| Noah Darden | Young Adam Rain |
| Bonnie Morgan | Marionette #114 |
| Stephen Anthony Bailey | Audience Member
(uncredited) |