| Joe Mantegna | David Rossi |
| Matthew Gray Gubler | Dr. Spencer Reid |
| A.J. Cook | Jennifer Jareau |
| Kirsten Vangsness | Penelope Garcia |
| Aisha Tyler | Dr. Tara Lewis |
| Daniel Henney | Matt Simmons |
| Adam Rodriguez | Luke Alvez |
| Paget Brewster | Emily Prentiss |
| Kim Rhodes | Assistant Director Linda Barnes |
| Moe Irvin | Chief Thomas Wheeler |
| Myles Bullock | Kevon Winters |
| Cile Turner-Borman | Edna |
| Salvador Benavides | M.E. Dr. Aristeo Caruso |
| Jay Linzy | Carl Kevork |
| Chiara Maya | Dr. Emily Hicks |
| Gregory Zarian | Walter Trudeau |
| David Abed | Overworked Doctor |
| Regina Williams | Tanesa Winters |
| Isaiah Russell-Bailey | Young Kevon Winters |
| Mario Perez | Ramon |
| Dahlia DaCosta | Dahlia |
| David Barrera | Mario Casteneda
(archive footage) (uncredited) |
| Tim Murdock | Tyler Roberts
(uncredited) |