| Joel McHale | Jeff Winger |
| Gillian Jacobs | Britta Perry |
| Danny Pudi | Abed Nadir |
| Yvette Nicole Brown | Shirley Bennett |
| Alison Brie | Annie Edison |
| Donald Glover | Troy Barnes |
| Ken Jeong | Ben Chang |
| Chevy Chase | Pierce Hawthorne |
| Betty White | Professor June Bauer |
| John Oliver | Professor Ian Duncan |
| Patton Oswalt | Male Nurse Jackie |
| David Jean Thomas | Chief Umbootu |
| Pat Crawford Brown | Pierce's Mom
(voice) |
| Amber Lancaster | Christine |
| Natalina Maggio | Hot Girl |
| Patrick O'Neil | Christine's Boyfriend |
| Bill Parks | Dude #1 |
| D.C. Pierson | Mark Millot |
| Justin Alston | Guy #2 |
| Brenda Arteaga-Walsh | Anthropology Student
(uncredited) |
| David Cohen | Teacher
(uncredited) |
| Alan Gray | Student in Cafeteria Line
(uncredited) |
| Alan Sepinwall | Student Watching Fight
(uncredited) |