| Nathan Fillion | Richard Castle |
| Stana Katic | Kate Beckett |
| Susan Sullivan | Martha Rodgers |
| Jon Huertas | Javier Esposito |
| Seamus Dever | Kevin Ryan |
| Tamala Jones | Lanie Parish
(credit only) |
| Molly C. Quinn | Alexis Castle
(as Molly Quinn) |
| Penny Johnson Jerald | Captain Victoria Gates
(credit only) |
| Dean Norris | Captain Peterson |
| Darren Pettie | Trapper John |
| Ian Kahn | Sal Martino |
| Jack Laufer | Mr. Davenport |
| Ty Upshaw | Dr. Huxtable |
| Noa Dori | Dr. Quinn |
| Jonah Wharton | Dr. Howser |
| Dawn-Lyen Gardner | Simone Jenkins |
| Barry Livingston | Abe |
| Aimee Parker | Grace |
| Frank Birney | Father McCaskey |
| Matt Medrano | Officer Monfriez |
| Tatum Shank | Officer Billups |
| Holly Daniels | Tanya Brandt |
| Quinn Friedman | Connor |