| Nathan Fillion | Richard Castle |
| Stana Katic | Kate Beckett |
| Susan Sullivan | Martha Rodgers |
| Ruben Santiago-Hudson | Captain Roy Montgomery |
| Molly C. Quinn | Alexis Castle
(as Molly Quinn) |
| Jon Huertas | Javier Esposito |
| Tamala Jones | Lanie Parish |
| Seamus Dever | Kevin Ryan |
| John Pyper-Ferguson | Dean Donegal |
| Ever Carradine | Mirielle Lefcourt |
| Ken Baumann | Ashley |
| Carmen Argenziano | Mario Rivera |
| Rick Hearst | Dr. Elliot Lefcourt |
| David Dean Bottrell | Byron H. Singer |
| Presciliana Esparolini | Teresa De La Torre |
| Christopher May | Arthur Sansone |
| Gattlin Griffith | Tyler Donegal |
| Paul Jerome | Robert Kincaid
(as Paul Eric Jerome) |
| J. Marvin Campbell | Man #2 |
| Claudia Choi | CSU Photo Tech |
| Al Pugliese | Pretzel Vendor |
| Danny Downey | BMX Rider #1 |
| Eddie Fiola | BMX Rider #2 |
| Steve Rizzo | Thomas Mestrick
(uncredited) |