| Nathan Fillion | Richard Castle |
| Stana Katic | Kate Beckett |
| Susan Sullivan | Martha Rodgers |
| Ruben Santiago-Hudson | Captain Roy Montgomery |
| Molly C. Quinn | Alexis Castle
(as Molly Quinn) |
| Jon Huertas | Javier Esposito |
| Tamala Jones | Lanie Parish |
| Seamus Dever | Kevin Ryan |
| Anita Barone | Janice Freeman |
| Robin Thomas | Alan Freeman |
| Erin Sanders | Rosie Freeman |
| Samantha Shelton | Vixen |
| Phil LaMarr | Dr. Holloway |
| Amy Hill | Alma |
| Jim Gleason | Dr. Barry Frank |
| Robert W. Arbogast | Morlock
(as Robert Arbogast) |
| Glenn Taranto | Douglas Lawson |
| Emily Bicks | Paige |
| Cole Coleman | Vampire /
Goth Club Patron
(uncredited) |
| Zoe Taylor | Elizabeth Dryden
(uncredited) |