| Nathan Fillion | Richard Castle |
| Stana Katic | Kate Beckett |
| Susan Sullivan | Martha Rodgers |
| Ruben Santiago-Hudson | Captain Roy Montgomery |
| Molly C. Quinn | Alexis Castle
(as Molly Quinn) |
| Jon Huertas | Javier Esposito |
| Tamala Jones | Lanie Parish
(credit only) |
| Seamus Dever | Kevin Ryan |
| Reed Diamond | Dr. Cameron Talbot |
| Diana Maria Riva | Det. Roselyn Karpowski |
| Arye Gross | M.E. Sidney Perlmutter |
| Elaine Hendrix | Melissa Talbot |
| Ivo Nandi | Teodor Hajek |
| Jeff Doucette | Super |
| Timi Prulhiere | Talbot's Nurse |
| Debi Mazar | Paula Haas |
| Griffin Cleveland | Zane Talbot |
| Francisco Viana | Manager |
| John Brantley Cole | NYPD Uniform |
| Heath Pennington | Hero Witness
(uncredited) |