| Andy Samberg | Jake Peralta |
| Stephanie Beatriz | Rosa Diaz |
| Terry Crews | Terry Jeffords |
| Melissa Fumero | Amy Santiago |
| Joe Lo Truglio | Charles Boyle |
| Chelsea Peretti | Gina Linetti |
| Andre Braugher | Raymond Holt |
| Dirk Blocker | Hitchcock |
| Joel McKinnon Miller | Scully |
| Aida Turturro | Maura Figgis |
| Carrie Armstrong | Roena Luesto |
| Nicole J. Butler | Sylvia Dunnes |
| Charmel Catrell | Martie |
| Joshua Feinman | Mystery Guest
(as Josh Feinman) |
| Paul Mabon | Officer Lou |
| Tricia McAlpin | Maggie |
| Ken Narasaki | Li Ongoing |
| Del Brown | Newbie Prisoner
(uncredited) |
| Jason Mantzoukas | Adrian Pimento
(archive footage) (uncredited) |
| Christopher Riordan | High Five Mourner
(uncredited) |