| Amybeth McNulty | Anne Shirley-Cuthbert |
| Geraldine James | Marilla Cuthbert |
| R.H. Thomson | Matthew Cuthbert |
| Cara Ricketts | Mary Lacroix |
| Dalmar Abuzeid | Sebastian 'Bash' Lacroix |
| Lucas Jade Zumann | Gilbert Blythe |
| Nicky Lawrence | Jocelyn |
| Lisa Codrington | Constance |
| Corrine Koslo | Rachel Lynde |
| Brian Paul | Dr. Ward |
| Kiawentiio | Ka'kwet
(as Kiawenti:io Tarbell: io Tarbell) |
| Brandon Oakes | Aluk |
| Dana Solomon | Oqwatnuk
(as Dana Jeffrey) |
| Wade Bogert-O'Brien | Oliver Thatcher |
| Richard Ausar Stewart | Bartender
(as Ausar Stewart) |
| Dalila Bela | Diana Barry |
| Kyla Matthews | Ruby Gillis |
| Jacob Ursomarzo | Moody Spurgeon |
| Glenna Walters | Tillie Boulter |
| Miranda McKeon | Josie Pye |
| Lia Pappas-Kemps | Jane Andrews |
| Ashleigh Stewart | Winifred Rose |
| Araya Mengesha | Elijah Hanford |
| Aymeric Jett Montaz | Jerry Baynard |
| Brian Tree | Minister |
| Ryan Kiera Armstrong | Minnie May Barry |
| Jonathan Holmes | Mr. William Barry |
| Helen Johns | Mrs. Eliza Barry |
| Philip Williams | Thomas Lynde |
| Joanna Douglas | Miss Stacy |
| Jacob Horsley | Charlie Sloane |
| Brooklyn Herd | Gillis Sister
(uncredited) |