| Amybeth McNulty | Anne Shirley-Cuthbert |
| Geraldine James | Marilla Cuthbert |
| R.H. Thomson | Matthew Cuthbert |
| Lucas Jade Zumann | Gilbert Blythe |
| Dalmar Abuzeid | Sebastian 'Bash' Lacroix |
| Taras Lavren | Nate |
| Shane Carty | Mr. Dunlop |
| Jonathan Holmes | Mr. William Barry |
| Helen Johns | Mrs. Eliza Barry |
| Dalila Bela | Diana Barry |
| Corrine Koslo | Rachel Lynde |
| Philip Williams | Thomas Lynde |
| Yanna McIntosh | Hazel |
| Aymeric Jett Montaz | Jerry Baynard |
| Stephen Tracey | Mr. Phillips |
| Christian Martyn | Billy Andrews |
| Cory Gruter-Andrew | Cole Mackenzie
(as Cory Grüter-Andrew) |
| Miranda McKeon | Josie Pye |
| Kyla Matthews | Ruby Gillis |
| Lia Pappas-Kemps | Jane Andrews |
| Glenna Walters | Tillie Boulter |
| Brian Tree | Minister |
| David Ingram | Mr. Andrews |
| Philip Graeme | Wealthy Man |
| Ngabo Nabea | Street Vendor |
| Max Rampino | Boy |
| Katelyn Wells | Mary Joe |
| Franckie Francois | Hammock Man
(as Franck Fon) |
| Kyle Meagher | Billy's Pal #1 |
| Daimen Landori-Hoffman | Billy's Pal #2 |
| Ryan Kiera Armstrong | Minnie May Barry |
| Janet Porter | Mrs. Andrews |
| Ella Jonas Farlinger | Prissy Andrews
(as Ella Jonas-Farlinger) |
| Jacob Horsley | Charlie Sloane |
| Jacob Ursomarzo | Moody Spurgeon |
| Katie Advani | Anne Acting Double |