| Amybeth McNulty | Anne Shirley |
| Geraldine James | Marilla Cuthbert |
| R.H. Thomson | Matthew Cuthbert |
| Dalila Bela | Diana Barry |
| Corrine Koslo | Rachel Lynde |
| Aymeric Jett Montaz | Jerry Baynard |
| Lucas Jade Zumann | Gilbert Blythe |
| Kyla Matthews | Ruby Gillis |
| Miranda McKeon | Josie Pye |
| Jacob Ursomarzo | Moody Spurgeon |
| Lia Pappas-Kemps | Jane Andrews |
| Deborah Grover | Josephine Barry |
| Helen Johns | Mrs. Eliza Barry |
| Ryan Kiera Armstrong | Minnie May Barry |
| Jacob Horsley | Charlie Sloane |
| Daniel Kash | Pawnbroker |
| Taras Lavren | Nate |
| Shane Carty | Mr. Dunlop |
| David Kirby | Doctor |
| Neil Foster | Bank Manager |
| Margaret Lamarre | Miss Rogerson |
| Kent Staines | Rollings |
| Tanya Bevan | Lady |
| Katelyn Wells | Mary Joe |
| Brian Tree | Minister |
| Emily Roman | Anne 'Double' |
| Brenda Bazinet | Jeannie |
| Nicholas Fry | Child in Choir #1
(uncredited) |
| Brooklyn Herd | Gillis Sister
(uncredited) |
| Teagan Rayne Sellers | Child in Choir #2
(uncredited) |
| Moira Walley-Beckett | Lady
(uncredited) |