| Kyle MacLachlan | Dale Cooper |
| Jay Aaseng | Drunk |
| Joe Adler | Roger |
| Mädchen Amick | Shelly Briggs |
| Dana Ashbrook | Deputy Bobby Briggs |
| David Bowie | Phillip Jeffries |
| Catherine E. Coulson | Margaret Lanterman (The Log Lady) |
| Owain Rhys Davies | Agent Wilson |
| Eamon Farren | Richard Horne |
| Sherilyn Fenn | Audrey Horne |
| Jay R. Ferguson | Special Agent Randall Headley |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Patrick Fischler | Duncan Todd |
| Robert Forster | Sheriff Frank Truman |
| Nathan Frizzell | Phillip Jeffries |
| Mark Frost | Cyril Pons |
| Pierce Gagnon | Sonny Jim Jones |
| Harry Goaz | Deputy Andy Brennan |
| Grant Goodeve | Walter Lawford |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Caleb Landry Jones | Steven Burnett |
| Sheryl Lee | Laura Palmer |
| Jennifer Jason Leigh | Chantal Hutchens |
| Peggy Lipton | Norma Jennings |
| James Marshall | James Hurley |
| Everett McGill | Big Ed Hurley |
| Clark Middleton | Charlie |
| Casey O'Neill | Skipper |
| John Pirruccello | Deputy Chad Broxford |
| Kimmy Robertson | Lucy Brennan |
| Wendy Robie | Nadine Hurley |
| Tim Roth | Gary 'Hutch' Hutchens |
| Rodney Rowland | Chuck |
| Carlton Lee Russell | Jumping Man |
| Malachy Sreenan | Bosomy Woman |
| Harry Dean Stanton | Carl Rodd |
| J.R. Starr | MC |
| Jessica Szohr | Renee |
| Jake Wardle | Freddie Sykes |
| Naomi Watts | Janey-E Jones |
| Alicia Witt | Gersten Hayward |
| Charlyne Yi | Ruby |
| Nae | Naido |
| Finn Andrews | The Veils |
| Sophia Burn | The Veils |
| Uberto Rapisardi | The Veils |
| Anthony Marcacci | |
| Jon-Thomas Butler | Black Lodge Woodsman |
| Christian Calloway | Black Lodge Woodsman |
| Lindsay Lane | Bang Bang Waitress |
| Damian Lund | Roadhouse Patron Biker |
| Alex Reyme | Bang Bang Bar Patron |
| Darryl Robert Small | Patron |