| Kyle MacLachlan | Dale Cooper |
| Elizabeth Anweis | Abbie |
| Chrysta Bell | FBI Agent Tammy Preston |
| Richard Beymer | Benjamin Horne |
| Scott Coffey | Trick |
| Ana de la Reguera | Natalie |
| Laura Dern | Diane Evans |
| Sherilyn Fenn | Audrey Horne |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Robert Forster | Sheriff Frank Truman |
| Pierce Gagnon | Sonny Jim Jones |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Ashley Judd | Beverly Paige |
| Luke Judy | Warden Murphy's Son |
| David Patrick Kelly | Jerry Horne |
| Sheryl Lee | Laura Palmer |
| Jennifer Jason Leigh | Chantal Hutchens |
| Sarah Jean Long | Miriam Sullivan |
| David Lynch | FBI Deputy Director Gordon Cole |
| Bérénice Marlohe | French Woman |
| Zoe McLane | Check-out Girl |
| Clark Middleton | Charlie |
| James Morrison | Warden Dwight Murphy |
| Johnny Ochsner | Bag-boy |
| Bill O'Dell | Kriscol |
| Wendy Robie | Nadine Hurley |
| Tim Roth | Gary 'Hutch' Hutchens |
| Harry Dean Stanton | Carl Rodd |
| Russ Tamblyn | Dr. Lawrence Jacoby |
| Grace Zabriskie | Sarah Palmer |
| Ruth Radelet | Chromatics |
| Adam Miller | Chromatics |
| Johnny Jewel | Chromatics |
| Nat Walker | Chromatics |
| Richard Garcia | Town Person |