| Kyle MacLachlan | Dale Cooper |
| Jane Adams | Constance Talbot |
| Dana Ashbrook | Deputy Bobby Briggs |
| Chrysta Bell | FBI Agent Tammy Preston |
| Brent Briscoe | Detective Dave Macklay |
| Michael Cera | Wally Brando |
| Richard Chamberlain | Bill Kennedy |
| David Dastmalchian | Pit Boss Warrick |
| David Duchovny | Denise Bryson |
| Miguel Ferrer | FBI Agent Albert Rosenfield |
| Robert Forster | Sheriff Frank Truman |
| Pierce Gagnon | Sonny Jim Jones |
| Brett Gelman | Supervisor Burns |
| Harry Goaz | Deputy Andy Brennan |
| James Grixoni | Deputy Jesse Holcomb |
| Michael Horse | Deputy Chief Tommy 'Hawk' Hill |
| Stephen Kearin | FBI Driver |
| Dep Kirkland | Police Chief Mike Boyd |
| Jay Larson | Limo Driver |
| Sheryl Lee | Laura Palmer
(credit only) |
| David Lynch | FBI Deputy Director Gordon Cole |
| Karl Makinen | Inspector Randy Hollister |
| James Morrison | Warden Dwight Murphy |
| Sara Paxton | Candy Shaker |
| John Pirruccello | Deputy Chad Broxford |
| Linda Porter | Lady Slot-Addict |
| Kimmy Robertson | Lucy Brennan |
| Al Strobel | Phillip Gerard |
| Ethan Suplee | Bill Shaker |
| Sabrina S. Sutherland | Floor Attendant Jackie |
| Jodi Thelen | Maggie
(as Jodee Thelen) |
| Naomi Watts | Janey-E Jones |
| Heather D'Angelo | Au Revoir Simone |
| Erika Spring Forster | Au Revoir Simone
(as Erika Forster) |
| Annie Hart | Au Revoir Simone |
| Tyler Malik | FBI Agent
(uncredited) |